The Social Institution Of Marriage In Jane Austen’s Society Essay examp

The Social Institution Of uniting In Jane Austens Society In the following essay I am going to closely examine the proposals of jointure Elizabeth by her full cousin Mr Collins, and aristocrat Mr Darcy.I am also going to compare and line of work the events of each proposal.In Jane Austens lifetime a womens side in society came first offfrom her parents and secondly when she married. Jane Austen shows themarked differences in distinguish frequently, as this was a major feature ofeveryday life in the 19th century. Men were seen as being far superiorto women as they were able to work and thus earn a small, and in lofty law of naturesuits a large fortune. Pride and Prejudice in some slipway mirrors JaneAustens own life, as her heart was broken at a tender age. JaneAusten gave her undying love to her hero Tom Lefroy, however the jeerproved in congruous as neither Jane or Tom had a sufficient income toallow them to live as man and wife. At only twenty Jane Austens reallove had c ome and gone and she never went on to love anyone else.Pride and Prejudice portrays the struggle for women to find compatiblemen, that not only satisfied their own need for love and adoration, moreover also suitors that meet their families best interests.In chapter nineteen it becomes clear to the proofreader that Mr Collins isinterested in Mrs Bennets daughters. He firstly questions theavailability of Jane but is told that she is soon to be engaged to MrBingley. After this come in back Mr Collins inquires about Elizabeth (thesecond eldest daughter of Mrs Bennet.) may I hope madam, for yourinterest with your fair daughter Elizabeth, when I crave for thehonour of a private audience with her in the course of this morning.Elizabeth move to inte... ...)within a family could have serious consequences on daughters and theireligibility for marriage. Many would keep spinsters or they wouldmarry outside their usual social circle. It was not unusual forcouples to become engaged in th eir late teens (with the lifeexpectancy at this time of approximately forty years it is perhaps not move that couples married young). Today many couples aremarrying in their thirties and marriage is not as fashionable orsocially vital. In the Bennets case as in that respect was no son and heirthere was the law of entailment whereby property was left to a malerelative. Therefore there was a real pressure for a daughter to marrythe successor so that property and wealth could remain within thefamily. Of course laws the likes of this do not generally exist today, andmarriages are a heart of love rather than a business proposition.

Health Risk Assessment Bnt’s Story Essay

To go with dignity should be a given. Death entrust present itself to everyone eventually and presumably no one in their decently mind would choose not to die with dignity. That being said why is it that so m either the great unwashed who would choose to die a bully destruction ar no allowed to. For nigh masses no last is a practiced decease, exclusively that is silly because as everyone knows death is infallible. No one can escape this fact. The controversy herein lies within the hatful raft who ar sanctioning or lingering with a termination illness that debilitates them such that they have no quality of life are not afforded the comfort of dying with dignity.It seems that this high merit is available to our sexual love pets, but not our belove family members. Aunt Bessie is forced to give the axeure unyielding both(prenominal)eration from here incurable pancreatic cancer. No amount of discommode medication is available to treat her pain and allow her to maint ain consciousness. She knows her age is limited and she does not feel she should have to wait bulge out the required in such a terrible state. At the same quantify the family pet Bassett hound is found to have a mass in her intestines. She cannot eat and barely moves as she is in so some(prenominal) pain.The veterinary has given the option of putting her to sleep or euthanizing her to put her out of her misery as she is misfortunate so. This sits well with most concourse. Aunt Bessie requesting euthanization though is out of the question. Why is the question that this paper testament attempt to say and why this is impose on _or_ oppress pull up stakes also be addressed? mercy killing Defined Euthanasia can bring about different faces to lot depending on the context in which it is used. Mention that good old dog and it is good as they no longer suffering.Change the identity to a soul and it is not so easily swallowed. No matter how it is understand the true meaning of the act is the same in either circumstance. Originating from the Greek terms eu (happy or good) and thanatos (death), mercy killing manner literally happy death or good death. (Le Baron Jr. , 1999). recess that definition stilt even push is subdivisions in the definition are voluntary/involuntary and active/passive Voluntary euthanasia is a death performed by another(prenominal) with the consent of the psyche being killed.Non-voluntary euthanasia is the provision of euthanasia to an incompetent person according to a deputys decision. Involuntary euthanasia is euthanasia performed without a competent persons consent. Passive euthanasia involves allowing a forbearing to die by removing her from artificial life substantiate systems such as respirators and feeding tubes or simply discontinuing medical treatments necessary to sustain life. Active euthanasia, by contrast, involves decreed steps to end the life of a patient, typically by deadly injection (Le Baron Jr. 1999) Euthanasia brings tranquility to death. In euthanasia the suffering prior to death is limited and death is entered more peacefully as opposed to lingering with pain and suffering.Presumably most people would want to end their journey in life without pain and suffering. A more compassionate death is a more desirable death. however to know what euthanasia in reality means requires more exploration into the attainment of this term. Practical Problems Euthanasia can bring about bad feeling to some people. After all when the term is used it is in relationship to death.In our culture and to numerous people death or speech production about death is taboo. This is an unfortunate fact. What is most unfortunate is that death is inevitable and therefore discussion regarding it should be more open. Essentially the problem with euthanasia is this taboo associated with it. Ethically euthanasia is an appropriate and well warranted act of good-will and consideration. Support of euthanasia wil l be provided within the context of this paper, and the potentiality negative issues will also be brought up. Ethics Argument For The honourable conventions that can justify euthanasia are many.This is because the premise of euthanasia is establish upon the idea of caring. This idea of caring is inherent to most care givers and is the line principle in euthanasia. Such acts of caring resonates through theses moralistic principleal principles and mannequins obeisance of persons, Virtue ethical motive, Utilitarian, Rights based ethics and ethics of caring. These frameworks or principles although many will all show auxiliary qualities for euthanasia. Respect of Persons In respect of persons the support of euthanasia is found in that the principle here is that individuals are afforded liberty and able to make their own decisions.When a person chooses not to suffer at the end of their life this is an autonomous decision. Respect for persons generally means respecting a clients self-sufficiency (Ethical Principles, 2011). That person in the eyeball of this principle is just in making that call. The respect of person principle sustains this in its foundation. The principle of respect for persons affirms the primary importance of allowing individuals to exercise their example salutary of self determination. To violate their ability to be self-determining is to treat them as less than persons. (Bennette-Woods, 2001). Therefore, this principle is then supportive of euthanasia when decided upon by an individual. Virtue Ethics With the act of euthanasia people are desire the good by allowing a good death. Suffering is not permitted to go on until death. Rather death is allowed to happen prior to a drawn-out battle with pain and suffering. Virtue ethics is doing good and for the chasten reason. Forcing people to live with pain and suffering would not be characterized as good. So presumably then allowing for euthanization is good.Because it is done in resp onse to alleviate further pain or suffering it satisfies the right reason aspect. In another view the dying patient is taking a virtuous stance. round dying patients consider it virtuous to spare friends and family the ordeal of witnessing a ho-hum process of degeneration (van Zyl, 2002, p. 19). This again satisfies the criteria for virtue ethics. Utilitarian In encompassing the utilitarian ethical frame work consideration must be make for the balance of the sterling(prenominal) good. Allowing a person to die a good death will promote the greatest happiness for both the person and for those remaining behind.Sure there will be sorrowfulness in that there is a loss with the person dying, but the greatest happiness will be in knowing that the person is no longer suffering and that the pain of their disease is not torturing them any longer. This satisfies part of the balance. The other part is on behalf of the individual who is suffering. There is much good in ending a life from pa in and suffering. A good death is much more desirable then a death of unrelenting pain and suffering. Therefore the balance of the greater good is established here and the utilitarian frame work is established. Right ground EthicsThe right to die is an inevitable right. Advocates of euthanasia argue that people have a right to make their own decisions regarding death, and that euthanasia is intend to alleviate pain and suffering. (Nargus, 2012) It is of the belief of many that all people hold this one true right. Dying is ultimate natural right. The patient has the right to make the decision about when and how they should die, based on the principles of autonomy and self-determination (Nargus, 2012). This alone substantiates the use of rights based ethics and upholds this as an ethical speckle for euthanasia.Ethics of Caring This is the final ethical stance that will be used to persuade the positive perspective of euthanasia. To care is the internality of this ethical principle. To care would be to not allow suffering with pain at the end of life. Compassion is a must in the ethics of caring and allowing pain and suffering to continue cannot be construed as compassionate. The ethic of care demands that we maintain conditions under which caring can flourish. (Bennette-Woods, 2001) How erupt to show a moxie of caring then by halt suffering, stopping pain and allowing for a good death.Ethics of caring is the soil for most nursing philosophies and can be equated to most nursing principles. bring off is not just in the physical sense, but the emotional sense as well. Care is delivered when euthanasia is allowed. Ethics Argument Against The origins proposing that euthanasia be allowed were provided and supported. Now a few counterarguments will be analyzed and provided. Kants original ethical framework intent was clearly against the use of euthanasia, but a newer vision of Kantian ethics could also be supportive of the act.However, the argument against it i n this framework will be what is focused on. Nonmaleficence framework can also be argued against euthanasia. This theory bases itself on doing no accidental injury and depending what is perceived as harm is how this argument can be made. Kantian Ethics A moral action is one that is performed solely for the utilization of meeting a moral obligation, and the action itself can only be judged moral in light of the intention behind it. If the intention produces death it cannot be a good intention. Death although now relieved of suffering is the outcome.With Kantian ethics the end result is not at question. The person no longer suffering perspective is not of any value. The point is euthanizing a person is morally wrong and the outcome (alleviating pain and suffering) has no bearing in the matter. This is why this argument was not used in the pros. The outcome has to matter. Nonmaleficence Ethics The principle of Nonmaleficence states that we should act in ways that do not inflict evil or cause harm to others. In particular, we should not cause avoidable or intentional harm. (Bennette-Woods, 2001)While some people feel that causing an earlier death as with euthanasia is harmful not everyone shares this view. Professional organizations have invoked professional obligations as an argument against support and participation in assisted suicide and euthanasia. (Ersek, 2004) . For those that do this could certainly be understood as argument against euthanasia. However, for others the harm is renowned in the continuance of a life of suffering. Catholic Moral tradition Each human life is considered sacred and deserving of a right to life.This is the position that the Catholic moral tradition stands behind. According to the Roman Catholic perspective, we are not obliged to ward off death at all costs, but we should not deliberately intervene to bring death about (Euthanasia a Catholic Perspective, 1987). The church goes on to say that the end of human life is not subjec t to a persons free judgment (Euthanasia a Catholic Perspective, 1987). This theory protests that as in birth death can only be implemented by God. However, even the church has come to make leave outions or loop holes that allows for euthanasia to happen. In the double effect principle medication can be given in large doses to relieve pain.The patient will submit to death as a result of this medication, and that is ok. As long as the intention is to relieve pain, not cause death the Catholic moral traditions is receptive to this. It seems as if the church has struggle d with this issue in the past and the best that they can deliver is this double effect doctrine. (This was actually developed in the 15th century). . Final Justification A slow, painful, infra dig(predicate) death is a fate that most of us would not worry on our worst enemies (Dyer, 1999) yet this fate are often offered to people, loved ones and family members.This categorically should not be. Euthanasia theories h ave been presented and supported both for and against the act. What it comes down to is how individuals understand things. Some religious factions are adamant that this act is stringently forbidden. This author does not share these views. There is no argument that it is wrong to kill someone. However, there has to be availabilities for exceptions. When an act is done for all the right reason it then becomes a just act. When a person is deemed termination and is some way suffering then this would create such an except.This is only one of an infinite number of except possibilities. Each parapraxis presenting itself must be evaluated for its ethical morality. Evaluation can be constituted by using the previous theories presented in favor of euthanasia. Should the case total itself to these proposed theories then it is indeed a just act. Pets are not made to suffer a miserable final existence (associated with the love of them) uncomplete should people. Loved ones, family, friend o r foe no one deserves to die suffering when a good death is an option.

Culturally Proficient Leadership

EDLA 626 Culture, renewing &038 Human Rights heathenishly Proficient Leadership The excite got(prenominal) Journey Begins in spite of turn outance Summary Part 1 Leadership as an Informed Personal Perspective Chapter 1 The Leadership Journey Begins Within getting counselling on reality mevery a nonher(prenominal) people in society still represent segregated lives based on race / ethnicity, class It is important that we look for inward to ourselves in order to understand our reactions to people ethnically several(predicate) than us. such(prenominal) understanding allows us to effectively teach other peoples children pickings a Look at My indoctrinate and What I See (and taket See) Schools and districts be moldd by social, political and economic forces non quickly apparent. Underlying the transparent elements of our drill communities are unapparent forces that make detail more impact on our educatees, schools and us. These in discernible historic forces contri stille to the common sense of privilege or deprivation experienced in our schools that impacts our students, parents and us. These forces are termed as equity issues and serve as the metaphorical elephant in the room that legion(predicate) pretend not to see. Are there justice Issues in Your School? Reaction to equity issues is dependent on ones own experiences as a student. Different experience produced for students of different cultural groups (past &038 present). regardless of personal experiences, a school leader and his/her vision benefits from knowledge of diachronic context of coming and equity issues. Two expressed determine not fully realized are universal public education with High School and impartial educational opportunities (ex. Only 27 states have compulsory education requirements to age 16). Progress toward universal education is tie in with advancement in faithful treatment and equal outcomes for students based on gender, race, ethnicity and a bleness is also evolving. Prominent researchers have pressed the issue for equity in schools for 2 generations. Confronting the Gaps as a Leadership Issue Leaders confront with challenge of leading schools in ways that provide equitable opportunities no matter of a students culture The mere composition of any school poses naturally challenges due to distinctions (culture, race, gender, socio-economic, achievement, etc. ). Leaders need to anticipate systemic access disparities of quality educational programs, experienced funding and equitable school funding otherwise the military position quo of violates will continue. More equitable funding alone does not even things out . . . must be accompanied by a change in the way many students are educated. Important question for leaders How do we meet the schoolman and social necessarily of students who come to school with a different come out of values, beliefs, socioeconomic experiences, demeanors, language and ability? NCLB as a Leadership scape NCLB can serve as a tool to support access and equity attacks. NCLB has made public aware of differential educational opportunity and achievement patterns that exist in our schools and communities. NCLB used as pretext to point out and address achievement crack cocaine issues. Such a gap is a multifaceted outcome measure of gaps in access to education. proceeding gaps differentiated by race, ethnicity, gender, class, etc. are being highlighted by the media. Definitions of bring out Terms Culture The set of practices and beliefs shared by members of a busy group that distinguish that group from other groups. ethnical Informancy Reflects our experience of having regulate cultural relationships that are authentic and trusting which allow for mutual discipline and feedback leading to personal growth. Demographic Groups Often used in schools in pace of subgroup. Dominant Culture A culture readily visible in a classroom and school which often benefits fr om treatment, attention and achievement while others may be hidden and not apparent and not receive equitable treatment or attain equal levels of success.. Equity Access to material and human resources in proportion to needs. Ethnicity Ancestral heritage and geography, common history and to some degree somatic appearance. National stemma A designation related to a persons rural area of birth and prior citizenship. Nativism The practice of valuing the rights of citizens born in this country oer those of immigrants (marginalization effort &038 attempt of immigrants). Race A concept developed by social scientists and misinterpreted and used by groups to characterize people by their physical features and to use those dissimilaritys to justify suppression of some while promotion of others. objurgation careful consideration of ones deportments, plans, values and assumptions in an effort to improve interpersonal and professional practice. Sexual Orientation An enduring, emot ional, romantic, sexual or affectional attraction to another person.It exists along a continuum and differs from sexual behavior because it refers to feelings and self-concept. Chapter 2 The Cultural advancement Leadership Lens Provides an overview of the tools of cultural advancement. Such will provide one with an important electron lens and knowledge for action. Getting Centered Educational gaps are our issue with at least 3 arguments being important prerequisites 1. We must acknowledge that educational gaps are historical and persistent. Although we inherited them, they cannot be ignored.The issue of academic underperformance of children of poverty and some visible minorities is not new information. 2. If gaps are to be closed, we must move fore to go out our values, behaviors, policies and practices of our schools. 3. We can make a difference when we pay attention to who students say they are and their needs before the needs of our own and that of the school system. Cult ural proficiency is A process that begins with us, not with our students or their communities A shift in thinking that moves us from viewing culture as problematic to embracing and esteeming culture. A lens through which we view our reference as educators A concept comprised of a set of quaternary relate tools to guide our practice. Cultural advance Is an Inside-Out Process Cultural proficiency is an inside-out process of personal and organizational change. It is who we are more than what we do. We are students of our assumptions well-nigh self, others and the context in which we work with others Fundamental to addressing educational gap issues is ones willingness and ability to examine yourself and your organization. Cultural proficiency provides leaders with a comprehensive, systemic structure to identify, examine and discuss educational issues in our schools. Cultural Proficiency Represents a Leadership Paradigm Cultural proficiency . . . a mindset for how we interact with all people regardless of primer . . . a worldview that carries explicit values, language and standards for effective personal interactions and professional practices . . . is a 24/7 approach to both our personal and professional lives . . . is not a set of independent strategies one learns to use with others. Educators who commit to culturally proficient practices represent a paradigmatic shift away from the status quo dominant group view. The paradigmatic shift moves from tolerating diversity to transformational commitment to equity. Cultural Proficiency as an Educational Leadership Lens The following four tools of cultural proficiency can be used as a template for a leaders personal and professional schooling 1. maneuver principles on which you can build an ethical and professional put up for effective cross-cultural communication and problem solving. 2.A continuum of behaviors that enables you to diagnose your values and behavior in such a way that you can better influ ence policies and practices of our profession. 3. inbred elements expressed in terms of standards of personal and professional take that serve as a framework for moveing to academic and social needs of the cultural groups in your school &038 community. 4. Barriers to this work framed in such a way that you are intentional in the use of the maneuver principles and essential elements. Effective educational leaders are clear about themselves relative to working with and leading culturally diverse communities.The Cultural Proficiency Toolkit Cultural proficiency is comprised of an interrelated set of 4 tools which provide the path for a leader to guide his personal and professional development in a cultural proficient manner. The Guiding Principles of Cultural Proficiency Guiding principles provide one with a moral philosophical framework to examine &038 under-stand beliefs about the education of students from cultural groups different from them. Guiding principles provide a f ramework of how the cultural diversity of students should inform professional practice when responding to student learning needs. A good place to see if school values align with predominant behaviors in the school is the mission / vision statement. The Cultural Proficiency Continuum Consists of 6 points. The first 3 (cultural destructiveness, cultural incapacity, cultural blindness) points focus on them as being problematic. The next 3 (cultural precompetence, cultural competence, cultural proficiency) focus on your practice as transformational leadership. 1. cultural destructiveness see the difference and eliminate it 2. cultural incapacity see the difference and make it appear wrong 3. ultural blindness see the difference and act like you wear offt see it 4. cultural precompetence see the difference and act but inconsistently in appropriateness 5. cultural competence see the difference and be inclusive 6. cultural proficiency see the difference and respond positively, eng age, adapt and commit to social justice / equity The Five Essential Elements of Cultural Competence These elements are standards for culturally competent values, behaviors, policies and practices I. Assessing Cultural noesis II. Valuing Diversity III. Managing the Dynamic of DifferenceIV. Adapting to Diversity V. Institutionalizing Cultural Knowledge Overcoming Barriers to Cultural Proficiency There are barriers to achieving culturally proficient actions. They exist together in combination not as isolated events. I. Resistance to Change II. Systems of burdensomeness III. A Sense of Privilege and Entitlement Cultural Proficiency is . . . An approach for ascend educators assumptions and values that undermine the success of some student groups A lens for examining how we include and honour the cultures and learning needs of all students in the educational process.

Gothic Satire Essay

The government issue of readiness is cause for much debate between teachers, students, and cites. However, it is obvious that readiness is not necessary every day. Homework should not be depute to students on a insouciant basis because of health issues, the aim of barrier and the problem with sequence management. So I suggest the teacher screwing assign the cookery twice a week, it is a way more than easy to let students done their work. To begin, daily homework is unnecessary because of health issues.Students who read too much homework good deal develop lamentable sleep habits many students pull all-nighters in an attempt to make up. some other health issue is the neglect of fresh air. Students show simple photo to sunlight can provide bodies with neccessay vitamins, but there is no sunlight at your desk in your bedroom Lastly, stress levels go through the jacket when faced with too much work in countries like China, overworked students capture a high suicide r ate. Evidently, too much homework can seriously damage your health. In addition, daily homework presents an unrealistic level of difficulty.If all teachers support daily homework, a student entrust have a heavy workload, with homework from four classes Some homework is scarce too difficult to be substituted alone a parent is not a teacher and should not be expected to bang all the answers. Finally, struggling students who face failure at groom and at home might choose to give up on school completely. A student who did not understand all day will fell even more discouraged when left alone to complete the work. Obviously, daily homework is simply too difficult. Finally, daily homework causes major(ip) problems when it comes to time.Many students need to work to supplement their family income or for posr=secondary cultivation there is no time for a student to manage a share-time job and homework. Valuable leisure time with family and friends also suffers when daily homework is a ssigned. Students need connections with the people who care about them in ordination to be emotionally and mentally healthy. With no time to simply straighten with those we love, we cannot form healthy relationships. Last but not least, students who have homework every day cannot join extracurricular activities such as sports and clubs.The educational system constantly stresses that a successful student is an active part of the school community, but a student with no free time has no time to join and make new friends. Thus, daily homework seriously compromises a students free time. In conclusion, collectible to problems such as good health, increased level of difficulty and lack of time, daily homework should not be assigned it is, quite simply, unrealistic. Teachers should keep in mind that they are developing future people, and people want a healthy mind, body and spirit.

Cell and Molecular Biology

Cell and Molecular Biology concluding Name Hadeel Binomar30 pts. Protein misfolding can be an aspect of several different kind-hearted disorders, including cystic fibrosis, Alzheimers disease, and atherosclerosis. M whatever times, the misfolded protein is a membrane protein.In fact, a pillow slip of diabetes insipidus results from a mutation in the G-protein-coupled vasopressin 2 sense organ that prevents the protein from reservation it to the cadre membrane A) Describe how this type of receptor would normally beat targeted to the membrane (from the beginning of translation) and B) indeed propose one mechanism by which the mutation could cause a lack of proper targeting.One of the close to important proteins target is G protein-coupled receptors, several signaling mechanisms depend on this type of receptor to change both internal and external stimuli to the intra boothular responses. Basically, one of the G couple receptor subfamilies is G-coupled Vasopressin-2- Receptor (V2 R), and this receptor is field of study through a strict quality control process at the endoplasmic reticulum, which presents the only correctly folded protein to gets through the secretory pathway.The elementary process of the V2 receptor is to activate the attached G protein that bound to the ? subunit then phosphorylated to GTP. The G protein couple receptor then activates the enzyme adenylate cyclase that catalyzes the reaction in the ER and forms bivouac from ATP. After that, cAMP acts as a second messenger and activates a protein kinase that phosphorylates the constitutional membrane proteins on the stall develop.Moreover, the secretory pathway organelles and the plasm membrane both atomic number 18 first introduced into the Endoplasmic reticulum, and the co-translationally proteins that can cross the ER be synthesized by the ribo slightly first then beats by chaperones to gets moves to the ER surface victimisation GTP that allows them to move toward the receptor t hen release it.The soluble proteins and the integral membrane proteins as I mentioned above, can be targeted through the ER and then translocated by the same mechanism.Further, several mutations occurring in the transmembrane region which impact the structure of the protein. These be multiple mutation sites, such(prenominal) as mutations occurring in the aminic acid residues which was acting as a causative agent for humanity disease.Also, there be other mutations occurring on the single site position that will affect the translation mechanism and cause more human diseases like cystic fibrosis, Alzheimers disease, and atherosclerosis that proves the function of V2R protein which plays important occasion in the translation during the protein folding process.One of the mechanism that may a reason for lacking the proper target protein is when the mutations of the CFTR constituent occurring and affect the function of the chloride ion channels and cause defect in the protein seq uences which lead to the production of diseases and misfolded of the proteins that are unable to recognize their functional destinations.Otherwise, Lack of the determine signals is another issue that prevents the protein from getting into the surface of the ER, also called the non-stop decay electric cellular pathway, because lack of this stop signals prevents mRNA from synthesis and translate the proteins, these distribute as a plosive mutation that inhibits the essential stop codons. 30 pts. Describe the try shown in figure 3 from the paper we discussed in class (Miller et al, 2003). (A)In your description, consider the following questions Why did they do it? How did they do it? What did they learn? (B) Diagram the results that you would seem to see in Lanes T, 1, 2, 3, and 4, if the amino acid signal DID in the protein Gap1p was mutated to stochastic amino acids and tell why and (C) Give two possible (different) results that talent occur if the amino acid signal LxxLE in B et1p was mutated to the amino acids DID (which are the signal in Gap1p).Diagram the results expected in Lanes T, 1, 2, 3, and 4 and develop why you predicted this result for each case.left20840701.A001.A4467225201739500The experimentation was performed to study the role of freight rate binding do of import of Sec24p in the process of protein sorting. To perform this study, both edition and wild types subunit Sec23/24p and Sec23/24L616W were harvested from microsomal membranes.The immunoblotting assay performed to quantify cargo molecules using radio-labeled secondary antibodies. Comparison of the mutant subunit with wild type illustrated disrespect of some molecules in mutant one, these molecules were cargo protein molecules. While some of them are packed in a way that similar to the wild-type. It meant that there were some signals that remained unaffected although mutation was there.Unexpectedly, it was found that in these unaffected molecules of mutation in Sec23/24p, packag e was better than the wild type. Further, they found the proteins that were highly affected had Bet1p and Gap1p/Sys1p chimera, because they tout ensemble depending on the Sys1p di-acidic of COPII vesicles. If the amino acid signal DID in the protein Gap1p was mutated to random amino acids, the resulting bands would be seen as in figure 1.A because the amino acids mogul be present in all the lanes. And Gap1p is required for di-acidic motifs to fuse together with the COPII vesicles at the mutated domain. Also, Sys1 peptide is not involved in Sec 23/24p because its mutation is not contained in a di-acidic motif. If the signal LxxLE was mutated to the DID, the expected Bet1p packaging would be shown in figure 2.A as not be seen in lanes containing Bet1p because the amino acid signal LxxLE does not exit later mutating to amino acids DID. The second probability for the Bet1p would be expected to bind with the vesicle for packaging when it interacts with di-acidic motifs which is need ed as shown in figure 2.B290512515621000581025113030004781550412752.B002.B6286501346202.A002.A20 pts. Explain the experiment shown in Figure 5C from the Shen et al. (2018) paper on the phosphorylation of CDC25 that we discussed in class.In checkition, be sure to also address the following A) why did they do this experiment (the central issue) B) what did they learn and C) what is another control experiment they could have do? D) Would these results change if you added a constitutively active form of LKB1 to the reaction? If so, how and why? A) Overall, the main purpose of this paper is to explain how the division of cells is highly regulated such that cells that fail to pass some specific stage-based tests cannot advance to the proceeding stages.In this particular experiment, HeLa cells were subjected to chemicals that are known to activate the enzyme AMPK. Also, the main catabolic processes that are involved to generate nada for cells to regeneration from G1/G2 were determined by the application program of radiochemical approaches, the experiment required to approve how highly conserved cellular energy sensor can significantly delay mitosis entry and energizing of AMP-activated protein kinase (AMPK).Further, defecate 1 family inactivates the cell cycle G2/M Phase which is controlled by Cdc3/cyclical B (mitotic cyclin-dependent kinase complex). They found that AMPK-dependents phosphorylation of CDC25C arranges a metabolic control point for the M- bod transition and the cell cycle var. G2.B) Also, they learned that suppression of Wee 1 or acute induction partially reinstates mitosis ingress in the context of activated protein kinase (AMPK). This experiment showed that when Phosphorylates Cdc25 is in a distributive and disordered state, it results into ultra-sensitivity in protein phosphorylation. C) For another control experiment, they might try exposing the U2OS (cell line) clones conditionally exhibiting Cdc25A etoposide.That will protagonist to test whether Cdc25A degradation is significant for the G2 control point or not.D) If they add an active form of LKB1 to the reaction, the results will not change, because in cells LKBI activity inhibits AMPK activation in response to different stimulations. Also, LKBI is lost upon consistent isolation and because no effects of phosphates observed.20 pts.Briefly describe the role of cyclin-CDK in the cell cycle and give an example of positive and negative control in this system. Also, describe how cell cycle regulation links to the stimulation of caspase-mediated cell death at the molecular level. In the beginning, the cell cycle depends on many an(prenominal) basic factors that control the regulation process starting from the signal organization molecules, growth factors and the Cyclin Dependent Kinases enzymes include the checkpoints which control the transition process surrounded by the cell cycle phases by binding to the cyclin proteins CDKs then phosphorylate other proteins to move out from one phase to another.The role of transcription factors is to turn on the signals for gene expression, DNA replication, and cell divisions. As an example of CDKs, cyclin-dependent kinase 1 CDK1 is a cell division cycle protein homolog 2, that has a primary role in human genus Cancer cells because CDK1 rather than any types of CDKs is fatal to the mutated version of MYC- dependent cancer that leads to a depletion of oncogenes like (Fos and Jun) in human cancer cells.Fos and Jun are feature forms of the transcription factor called AP1 that activates the delayed response genes such as cyclin D and CDK4. Recent studies reveal that the reason for the MYC breast cancer cells duplation is targeting CDF1 exhibit any other CDKs cell lines. Also, CDK1 inhibition can control and target the cancer cells in human and both phosphorylation and expression of MYC during the cell cycle process.To novitiate intracellular signaling pathways and draw the cell cycle entry, mitogens sub stance bind to the cell surface receptor with the activation form of GTPase Ras that activates present kinase cascade. That will lead to the expression of encoding gene of the transcription regulative protein like MYC. Moreover, E2F transcription factor is a target for cyclin D and CDKs that stimulate proteins expression to initiate S phase, also E2F regulated by the tumour suppressor gene gene Rb (Retinoblastoma protein).At early G1 Phase, Rb protein unite with E2f to form the histone deacetylases protein that remains chromatic condensed, then cyclin kinase phosphorylates Rb protein, causing it to separate from E2F. That allows recruitment of histone acetylates, which decondense the chromatin and helps transcription complexes to form in G1 and S phase and to form a positive feedback.For the negative control, if there are no growth factors present to stimulate the synthesis of Cyclin D in the peeled cell, Rb phosphorylated will turn off, and Rb will rebind to E2F, then the cel l cycle will stop and that will lead to many negative results like prevent it to bind with DNA, or DNA disability and cells will return to the G0 phase.Apoptosis is a consequence of DNA disparage if the damage is not repaired, the cell cycle will divert towards apoptosis, otherwise, if the cell has DNA defects and fail to undergo apoptosis, that will change to the cancer cell. G1 phase regulators such as P53 and E2F are essential to promote the cell regulations and top any damage or abnormal changes during the cell cycle.P53 has a primary function to prevent any mutation in DNA during cell progression, so any defect in P53 will lead to cancer, and its commonly mutated in cancer cells. Further, Rb protein considers as a tumor suppressor and also promotes apoptosis. Additionally, most human cancers have inactive Rb protein, every mutated Rb or the non-phosphorylated (inactivated form) of Rb protein

Bellboy

Lewis also appears in a speaking role playing himself escorted by a large entourage, as his bellhop counterpart simultaneously emerges from a crowded elevator. Principal photography took place from February 8 to March 5, 1960 and label Jerry Lewiss debut as a director. Filming took place at the Fontainebleau Hotel in Miami Beach, Florida Lewis would film during the day and perform in the nightclub at night. l in advance he began, Lewis consulted his friend Stan Laurel about the script.Since Laurel had worked in unruffled films and was familiar with pantomime, he offered suggestions. It is unknown if Lewis actually used any of Laurels ideas in the production. 2 But it is believed Lewis paid homage to the comic by naming his component Stanley after him. A Stan Laurel-like character also appears throughout the story, portrayed by writer and impressionist Bill Richmond. The film marked an pioneering use of a video assist system, providingLewis a way to see the action counterbalance though he was in the scene. 3 Paramount wanted to have a Jerry Lewis movie for summer going away (in North America). The movie that it wanted to release was Cinderfella, which had finished shooting in December 1959. Lewis wanted to hold underpin the release of that movie for the Christmas 1960 holiday and Paramount only agreed if Jerry could take over another movie for summer. Therefore, while playing an engagement in Miami Beach, Lewis came up with this.